Antigen

Antigen Class

TFs and others

Antigen

Nkx2-1

Cell type

Cell type Class

Others

Cell type

Thyroid Gland

MeSH Description

A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.

Sample

source_name

thyroid

tissue

thyroid

age

1 month

strain

C57BL/6

genotype

WT

diet

Low Iodine Diet (LID, TD.95125, Harlan)

antibody

NKX2.1 Abcam, ab76013

Sequenced DNA Library

library_name

GSM6318146

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Immunoprecipitated chromatin was washed and ChIPed-DNA was eluted, reverse cross-linked, and DNA fragments purified using PCR purification kit form Qiagen. NEXTflex DNA-Seq kit from PerkinElmer was used.

Sequencing Platform

instrument_model

Illumina MiSeq

mm10

Number of total reads

27967019

Reads aligned (%)

89.8

Duplicates removed (%)

22.8

Number of peaks

30599 (qval < 1E-05)

mm9

Number of total reads

27967019

Reads aligned (%)

89.5

Duplicates removed (%)

22.7

Number of peaks

31601 (qval < 1E-05)