Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Nkx2-1

Cell type

Cell type Class
Others
Cell type
Thyroid Gland
MeSH Description
A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.

Attributes by original data submitter

Sample

source_name
thyroid
tissue
thyroid
age
1 month
strain
C57BL/6
genotype
WT
diet
Low Iodine Diet (LID, TD.95125, Harlan)
antibody
NKX2.1 Abcam, ab76013

Sequenced DNA Library

library_name
GSM6318146
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitated chromatin was washed and ChIPed-DNA was eluted, reverse cross-linked, and DNA fragments purified using PCR purification kit form Qiagen. NEXTflex DNA-Seq kit from PerkinElmer was used.

Sequencing Platform

instrument_model
Illumina MiSeq

mm10

Number of total reads
27967019
Reads aligned (%)
89.8
Duplicates removed (%)
22.8
Number of peaks
30599 (qval < 1E-05)

mm9

Number of total reads
27967019
Reads aligned (%)
89.5
Duplicates removed (%)
22.7
Number of peaks
31601 (qval < 1E-05)

Base call quality data from DBCLS SRA